#bacterialDNA

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#DNA#bacterialdna#science#bacterialDNA#bacteria#dna#microbiology#scientist#womeninscience#dnaextraction#phd#biology#molecularbiology#educatetheyoung#empoweringwomen#postdoc#nucleicacid#stemwomen#stemgirls#womeninstem#molbio#labscissors#girlsinscience#humanDNA#postdocworld#stemphd#nerdsofinstagram#lifeofapostdoc

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Phenol:Cloroform extraction: DNA isolation! Samples are mixed with a lysis buffer and the phenol:chloroform. After mixing it, the samples are centrifuged and end up in a separation of the phases. The upper layer (liquid) consist of the DNA, which will be transferred into a new tube with isopropanol, to precipitate. #dna #samples #experiment #phenolchloroform #phenol #chloroform #centrifugation #laboratorium #gloves #dnaisolation #dnaextraction #labwork #work #science #scientist #research #bacteria #bacterialdna #isolation #phd #phdlife #womeninscience #savetheworld


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It's been a while since I posted something "school related".
These are the results of chromosomal and plasmid DNA extraction from E.coli, visualized with UV light💉💊 #science #scientist #microbiology #biotechnology #student #DNA #dnaextraction #ecoli #bacteria #bacterialdna #ilovescience #sciencelover #agarose #agarosegel #gelelectrophoresis #electrophoresis #uv #uvlight


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🌞🔎Light allows magnification #sciart


9

Lab scissors continued --- Dr. Mary McMillan

In my last post I showed you my lab scissors – better known in the business as restriction enzymes.
So I thought I should follow up and show you what they do!
This is a picture of an agarose gel – used to separate fragments of DNA according to their size. Each glowing band on the gel is a group of DNA molecules all of the same size. In the lane on the left I have loaded a DNA ladder, which is a collection of DNA fragments of known sizes. We can use this like a ruler to measure the size of other fragments of DNA on the gel.
In the middle lane I have loaded one of my DNA fragments that hasn’t been exposed to my restriction enzyme. In the lane on the right I have the same DNA sample, except I have mixed the DNA with one of my restriction enzymes. The enzyme has cleaved the DNA, resulting in shorter fragments.
My main use for this technique is to look for genetic variation within certain regions of DNA. If there are changes to DNA, it can change the restriction sites, or places where the enzymes will cut DNA. Sometimes it will mean there is no longer one of these sites, and the DNA will remain uncut. Other times changes to DNA can change where the restriction sites are, moving them closer together or further apart, so that I get different size fragments after the digestion. By looking at the different patterns of bands on a gel like this I can figure out if different people have the same version, or different versions of a gene. So my lab “scissors” are pretty useful!

#restrictiondigestion #restriction #molecularbiology #restrictionenzymes #labscissors#DNA #molbio #womeninstem#stemgirls #stemwomen#womeninscience #DNA #nucleicacid#educatetheworld #educatetheyoung#empoweringwomen #girlsinscience#bacterialDNA #humanDNA#nerdsofinstagram #instascience#theaddicitivebrain #sciency#steminist #stemworld #stemphd #phd#postdoc #postdoclife #lifeofapostdoc#postdocworld


3

Lab scissors by Mary McMillian

I thought today I would show you some of my lab scissors. Ok, I know that these probably don’t look like scissors to most people, but to a molecular biologist these are scissors for cutting DNA.
These are just a few of my restriction enzymes that I have in the lab. Restriction enzymes are cool little proteins that cut up DNA. Each of these enzymes here recognises different sequences in DNA, called restriction sites. When they find those sequences in a piece of DNA they will cut the DNA at that position. Some restriction enzymes cut straight through the DNA, leaving what we call a blunt end. Other enzymes make staggered cuts in the DNA, leaving single stranded overhangs that we call sticky ends.

This is particularly useful if you are a molecular biologist, because you can cut pieces of DNA from different sources with the same enzyme, and they will then have matching ends that can be stuck (or ligated if you want the technical term) back together. This is how we create recombinant DNA. In my case I often use restriction enzymes to cut some human DNA and some bacterial DNA, and then stick the two together so that I can get bacteria to express human genes.
PS. Stay tuned and in my next post I’ll show you how these restriction enzymes work in practise :) Thank you Mary, for explaining about the restriction enzymes. We look forward to your next post about the working mechanism of these scissors.

#restrictionenzymes #labscissors #DNA #molbio #womeninstem #stemgirls #stemwomen #womeninscience #DNA #nucleicacid #educatetheworld #educatetheyoung #empoweringwomen #girlsinscience #bacterialDNA #humanDNA #nerdsofinstagram #instascience #theaddicitivebrain #sciency #steminist #stemworld #stemphd #phd #postdoc #postdoclife #lifeofapostdoc #postdocworld #australianscientist #australia


3

Although the lowest land on earth is named the Dead Sea, it isn’t actually completely dead. Remarkably, there are actually bacteria that thrive in the salination of the Dead Sea (322.6 gm salt/litre of water)! This limited variety of micro-organisms (halophylic) have developed complex mechanisms to adjust to the hostile environment.
💧
This is just one impressive feat of a single super-organism part of the enormous, worldwide, interacting, loose network of bacteria. There are many more astonishing things that large varieties of bacteria can accomplish.
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So before you go to medical battle with bacteria (sanitisation, antibiotics, man-made probiotics, etc.), please consider a more symbiotic relationship with these genetically intelligent and powerfully strong microbes. Do the world a favour and treat bacteria like your family. Perhaps get a little closer with them and embrace them all over your body, inside and out.
💞
#DontFuckWithMicrobes #MahaLavana #MagnesiumAbsorption


16

Scientists already have managed to translate all of #Shakespeare’s sonnets into #DNA. #GeorgeChurch, a #geneticist at #Harvard and one of the authors of the new study, recently #encoded his own book, “#Regenesis,” into #bacterialDNA and made 90 billion copies of it.#crispr


0

In case anyone was wondering how I'm spending my spring break... DNA extractions! A very repetitive and tedious process that takes me about 2 hours for every 8 samples. Here I am removing microfilters from tubes that have just come out of the centrifuge and using a solution to rinse off the purified DNA. #ScienceIsFun #WomenInScience #BacterialDNA #DNA #👩🏼‍🔬


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Kalau kamu sukanya calon dokter yang menyuntikkan antibiotik, apalah aku calon scientist yg cuma menyuntikkan DNA dari bakteri yg mampu menghasilkan antibiotik.. *Tsaaahhhh 😂😂😝😝
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#janganlupabahagia #senyumuntukbahagia #scientist #scienceforlife #microbiology #microbiologist #biologist #biotechnology #geneticsengineering #electroforesis #lactidacidbacteria #bacterialdna #antimicrobial #antibiotics


0

Oh, you thought DNA was something cool AND pretty? Sorry to disappoint but... well, no, it's not. 😃
#science #isolated #DNA #cool #molecularbiology #MasarykUniverzity #muni #lab #bacteria #bacterialDNA #lookslikesomeonespatinit #ew


1

Today is DNA extraction day! See that little white spot on the bottom of the tube? That's our bad boy! :) #DNA #dnaextraction #dnapellet #microtube #science #bacterialdna #qiagen #theknittingscientist


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